| TESTS | GUIDELINE |
|---|---|
| Aquatic primary producers: Freshwater algae and cyanobacteria, growth inhibition test: | |
| Green algae: Desmodesmus subspicatus and Pseudokirchneriella subcapitata
Blue Green algae: Anabaena flos-aquae Diatoms: Navicula pelliculosa | OECD 201, ISO 8692, DIN 38412-L33 |
| Seawater/estuaries micro-algae: | |
| Phaeodactylum tricornutum, 72h growth inhibition test | ISO 8692, OECD 201 |
| Higher aquatic plants: | |
| Lemna sp., growth inhibition test | OECD 221, ISO 20079 |
| Spirodela polyrhiza, 3 days growth inhibition test | ISO 20227 |
| Aquatic invertebrates | |
| Crustaceans: | |
| Daphnia magna, acute immobilisation test | OECD 202, ISO 6341, DIN 38412-L30 |
| Daphnia magna, reproduction test | OECD 211, ISO 10706 |
| Chironomus sp., acute immobilisation test | OECD 235 |
| Thamnocephalus platyurus, 24h mortality test | ISO 14280 |
| Ceriadaphnia dubia, 24h mortality test | USEPA method |
| Artemia franciscana, 24h mortality test | Standard method |
| Heterocypris incongruens, 6 days mortality and growth inhiition test | ISO 14371 |
| Thamnocephalus platyurus, 30-60 min particle ingestion inhibition test | ISO 14380 |
| Rotifers: | |
| Brachionus calyciflorus, 24h mortality test | ISO 19827 |
| Brachionus calyciflorus, 48h reproduction inhibition test | ISO 20666 |
| Brachionus plicatilis, 24-48h mortality test | ISO 19820 |
| Protozoans: | |
| Tetrahymena thermophila, 24h reproduction inhibition test | OECD method |
| Bacteria: | |
| Vibrio fischery, 30 min luminescence inhibition test | Standard method |
| Terrestrial plants | |
| Seedling emergence and seedling growth test | OECD 208 |
| Vegetative vigour test | OECD 227 |
| Measurement of the inhibition of root growth | ISO 11269-1 |
| Chronic toxicity in higher plants | ISO 22030 |
| Terrestrial invertebrates | |
| Earthworm acute toxicity test | OECD 207, ISO 11268-1 |
| Earthworm reproduction test | OECD 222, ISO 11268-2 |
| Earthworm avoidance test | ISO 17512-1 |
| Enchytraeid reproduction test | OECD 220, ISO 16387 |
| Collembolan reproduction test | OECD 232, ISO 11267 |
| Collembolan avoidance test | ISO 17512-2 |
| Predatory mite reproduction test | OECD 226 |
| Soil arthropod community test | Chelinho et al. 2014 |
| Bioaccumulation in terrestrial oligochaetes | OECD 317 |
| Terrestrial field and semi-field tests | |
| Terrestrial Model Ecosystems (TMEs) | Schäffer et al. 2009 |
| Determination of effects on earthworms in field situations | ISO 11268-3 |
| Breakdown of organic matter in litter bags in the field | OECD GD 56 |
| Field studies with other soil invertebrates (enchytraeids, mites, collembolans and nematodes) | ISO 11268-3, ISO 23611-2, ISO 23611-3, ISO 23611-4, ISO 23611-6 |
| Bait lamina tests to assess feeding activity of soil organisms | ISO 18311 |
| Bees | |
| Acute oral toxicity test with honeybees (Apis mellifera): effects on adult workers | OECD 213 |
| Acute contact toxicity test with honeybees (Apis mellifera): effects on adult workers | OECD 214 |
| Chronic oral toxicity test (10-day feeding) with honeybees (Apis melifera): effects recently emerged bees | OECD 245 |
| Acute oral toxicity test with honeybees (Apis mellifera): effects on larvae after single exposure | OECD 237 |
| Chronic oral toxicity test with honeybee (Apis mellifera): effects on larvae after repeated exposure | OECD 239 |